Recombinant eel FGF-2 protein 154 aa (bFGF/basic FGF) for the development of optimised serum-free culture media for species-specific European (Anguilla anguilla), Japanese (Anguilla japonica) and American (Anguilla rostrata) eel in cellular agriculture protocols and veterinary research applications. This longer form of FGF-2 is used in comparative cell culture media optimisation studies alongside Qk106, the 145aa form of eel FGF-2.
FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary eel cell culture. Receptor binding affinity and efficacy may differ depending on each species. Using a species-specific growth factor enhances receptor binding affinity, resulting in a lower concentration required in culture.
Eel FGF-2 is a high purity 17.2 kDa FGF-2 / bFGF protein, animal origin-free (AOF) and carrier-protein free (CF).
- High purity protein specific to European (XP_035281190.1), Japanese, and American (XP_064200074) eel
- >98%, by SDS-PAGE quantitative densitometry
- Source: Expressed in E. coli
- 17.3 kDa (Monomer)
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in Cambridge, UK
- Lyophilised from Tris/NaCl/CyS/mannitol
- Resuspend in water at >100µg/mL, prepare single-use aliquots, add carrier protein if desired, and store frozen at -20°C or -80°C